kolter's gonna be jealous

Dr Legostar · Post by **Dr Legostar** » Wed Jul 12, 2006 8:35 am

I'm working with P32 today!

Post by **Kisai** » Wed Jul 12, 2006 8:42 am

Is that like something explosive?

Rkolter · Post by **Rkolter** » Wed Jul 12, 2006 8:48 am

It's a radioactive isotope of Phosphorus. A big beta emitter.

I AM jealous - radioactive AND explosive on contact with water.

So cool. I want some!

http://www.unh.edu/ehs/radsafety/Fact_Sheets/P-32.html

Shishio · Post by **Shishio** » Wed Jul 12, 2006 9:12 am

Better not let RKolter, or any other of the resident mad scientists salivate on it, then.

Netpoet · Post by **Netpoet** » Wed Jul 12, 2006 9:37 am

Lego *IS* one of the resident mad scientists! FLEE!!

Col · Post by **Col** » Wed Jul 12, 2006 9:50 am

The end is nigh!

*pops some popcorn*

TheSuburbanLetdown · Post by **TheSuburbanLetdown** » Wed Jul 12, 2006 10:34 am

*steals popcorn*

Dr Legostar · Post by **Dr Legostar** » Wed Jul 12, 2006 10:36 am

well it's P32 as attached to ATP, so it's not explosive on contact with water, at least it wasn't when i put the water on.

Rkolter · Post by **Rkolter** » Wed Jul 12, 2006 10:39 am

PeppermintAfterlife wrote:*steals popcorn*

*sneaks in and sprinkles crushed halite with magnesium carbonate and iodine onto the popcorn while nobody is looking*

*sneaks off giggling evilly*

Carlin · Post by **Carlin** » Wed Jul 12, 2006 10:44 am

rkolter wrote:
PeppermintAfterlife wrote:*steals popcorn*
*sneaks in and sprinkles crushed halite with magnesium carbonate and iodine onto the popcorn while nobody is looking*

*sneaks off giggling evilly*

...dammit... now I need to google until I figure out what it is you just did.

Dr Legostar · Post by **Dr Legostar** » Wed Jul 12, 2006 10:49 am

it is indeed diabolical, and i don't have to google it.

Rkolter · Post by **Rkolter** » Wed Jul 12, 2006 11:16 am

legostargalactica wrote:well it's P32 as attached to ATP, so it's not explosive on contact with water, at least it wasn't when i put the water on.

Ingenious - a biological delivery system for radioactive explosives! Now you just need an enzyme that snips the P32 off the ATP.

RemusShepherd · Post by **RemusShepherd** » Wed Jul 12, 2006 11:22 am

Carlin wrote:
rkolter wrote:
PeppermintAfterlife wrote:*steals popcorn*
*sneaks in and sprinkles crushed halite with magnesium carbonate and iodine onto the popcorn while nobody is looking*

*sneaks off giggling evilly*
...dammit... now I need to google until I figure out what it is you just did.

*sneaks in while Carlin's not looking, and drizzles bovine triglyceride biolipids on top*

Dr Legostar · Post by **Dr Legostar** » Wed Jul 12, 2006 11:23 am

rkolter wrote:
legostargalactica wrote:well it's P32 as attached to ATP, so it's not explosive on contact with water, at least it wasn't when i put the water on.
Ingenious - a biological delivery system for radioactive explosives! Now you just need an enzyme that snips the P32 off the ATP.

um.. actually a lot of things do that.. it's kinda the point, cause this way it incorporates into the DNA and radioactively labels it.

it's just that when it incorporates/clips it's as PO4 not pure P

Rkolter · Post by **Rkolter** » Wed Jul 12, 2006 11:40 am

legostargalactica wrote:
rkolter wrote:
legostargalactica wrote:well it's P32 as attached to ATP, so it's not explosive on contact with water, at least it wasn't when i put the water on.
Ingenious - a biological delivery system for radioactive explosives! Now you just need an enzyme that snips the P32 off the ATP.
um.. actually a lot of things do that.. it's kinda the point, cause this way it incorporates into the DNA and radioactively labels it.

it's just that when it incorporates/clips it's as PO4 not pure P

Bah. Where's the fun in that?

Say, P32 is pretty darn radioactive - half life of 14 days or so. Just how much of that are you giving er... whomever? I'm surprised it isn't doing some substantial damage to the DNA it's incorporating into.

Dr Legostar · Post by **Dr Legostar** » Wed Jul 12, 2006 11:50 am

rkolter wrote:
legostargalactica wrote:
rkolter wrote: Ingenious - a biological delivery system for radioactive explosives! Now you just need an enzyme that snips the P32 off the ATP.
um.. actually a lot of things do that.. it's kinda the point, cause this way it incorporates into the DNA and radioactively labels it.

it's just that when it incorporates/clips it's as PO4 not pure P
Bah. Where's the fun in that?

Say, P32 is pretty darn radioactive - half life of 14 days or so. Just how much of that are you giving er... whomever? I'm surprised it isn't doing some substantial damage to the DNA it's incorporating into.

um... not giving to someone, mixing with known DNA to see if certain DNA binding proteins are present in various samples, and how much they are.

http://en.wikipedia.org/wiki/Gel_shift_assay

is kinda what i'm doing.

Rkolter · Post by **Rkolter** » Wed Jul 12, 2006 12:20 pm

So... you have some P32, but it's bound to ATP and it doesn't explode in water. And you have an enzyme that snips the P32, but it does so with an oxidized form that doesn't explode in water. And you aren't actually giving it to anyone.

You have a potentially explosive, radioactive chemical and you aren't doing anything even vaguely mad-scientistlike with it.

Col · Post by **Col** » Wed Jul 12, 2006 12:28 pm

*doesn't want his popcorn back*

Grabmygoblin · Post by **Grabmygoblin** » Wed Jul 12, 2006 12:34 pm

RemusShepherd wrote:
Carlin wrote:
rkolter wrote: *sneaks in and sprinkles crushed halite with magnesium carbonate and iodine onto the popcorn while nobody is looking*

*sneaks off giggling evilly*
...dammit... now I need to google until I figure out what it is you just did.
*sneaks in while Carlin's not looking, and drizzles bovine triglyceride biolipids on top*

*thinks about for a long long long time* ...aaah... yes... no wait... yes!! I get it!

Dr Legostar · Post by **Dr Legostar** » Wed Jul 12, 2006 6:10 pm

rkolter wrote:So... you have some P32, but it's bound to ATP and it doesn't explode in water. And you have an enzyme that snips the P32, but it does so with an oxidized form that doesn't explode in water. And you aren't actually giving it to anyone.

You have a potentially explosive, radioactive chemical and you aren't doing anything even vaguely mad-scientistlike with it.

well it was at work, and not in my spare time. you're still jealous though, i bet.